Journal: Neuron

Article Title: Endocannabinoid Signaling Collapse Mediates Stress-Induced Amygdalo-Cortical Strengthening

doi: 10.1016/j.neuron.2019.12.024

Figure Lengend Snippet: Key Resource Table

Article Snippet: Also see . table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit-α-cFOS Abcam 190289 RRID: AB_443538 Alexafluor 488 Donkey-α-Rabbit Invitrogen A21206 RRID: AB_221544 Rabbit-α-DAGLα Gift from Ken Mackie N/A Bacterial and Virus Strains AAV5-CMV-fDIO-Cre-mNeonGreen-wPRE This paper VB180530-1030aad rAAV2-CAG-tdTomato ( Chan et al., 2017 ) RRID: Addgene_59462 AAV5-CaMKII-ChR2(H134R)-eYFP-wPRE ( Lee et al., 2010 ) RRID: Addgene_26969 rAAV2-EF1a-mCherry-IRES-Flpo (Fenno et al., 2014) RRID: Addgene_55634 AAV5-CMV-Cre-eGFP-SV40 Gift from James M. Wilson RRID: Addgene_105545 rAAV2-pmSyn1-EBFP-Cre ( Madisen et al., 2015 ) RRID: Addgene_51507 AAV5-EF1a-DIO-ChR2(H134R)-eYFP-wPRE Gift from Karl Deisseroth RRID: Addgene_20298 AAV5-hSyn-DIO-hM3D(Gq)-mCherry ( Krashes et al., 2011 ) RRID: Addgene_44361 rAAV2-hSyn-GCaMP7f-wPRE ( Dana et al., 2016 ) RRID: Addgene_104488 Biological Samples Chemicals, Peptides, and Recombinant Proteins Rimonabant Cayman Chemical 9000484 DO34 Glixx Laboratories GLXC-09757 JZL184 Cayman Chemical 13158 CP55,940 Cayman Chemical 13608 PF3845 Cayman Chemical 13279 NESS0327 Cayman Chemical 10004184 CNO-HCl Cayman Chemical 25780 Deposited Data Experimental Models: Cell Lines Experimental Models: Organisms/Strains CB1 f/f mice Dr. Eric Delpire N/A DAGL f/f mice Dr. Sachin Patel N/A C57 WT mice Jackson Laboratory IMSR_JAX000664 Oligonucleotides Recombinant DNA Software and Algorithms Prism 6 Graphpad www.graphpad.com pClamp10.5 Molecular Devices www.moleculardevices.com MATLAB Mathwords www.mathworks.com ANY-maze Stoelting Co www.Stoelting.com Video Freeze Med associates www.med-associates.com Other Fear Conditioning Chamber Med associates MED-VFC-SCT-M Elevated Plus Maze San Diego Instruments Elevated Zero Maze San Diego Instruments Open in a separate window Key Resource Table

Techniques: Virus, Recombinant, Software

Journal: Neuron

Article Title: Orchestrating Opiate-Associated Memories in Thalamic Circuits

doi: 10.1016/j.neuron.2020.06.028

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-c-Fos Santa Cruz Biotechnology SC-52G Donkey anti-rabbit 647 Invitrogen A-31573 Bacterial and Virus Strains AAV1.hSyn.eGFP.WPRE.bGH University of Pennsylvania vector core or Addgene Addgene: 50465-AAV1 AAV1.hSynp.hChR2(H134R)-eYFP.WPRE.hGH University of Pennsylvania vector core or Addgene Addgene: 26973-AAV1 AAV1.CAG.ArchT.GFP.WPRE.SV40 University of Pennsylvania vector core or Addgene Addgene: 29777-AAV1 AAV8.hSynp.hM4D-mCherry This manuscript N/A rAAV2-retro-CAG.nls-mRuby3 This manuscript N/A AAV9-hEF1a-DIO-hM4D-mCherry-WPRE-pA Taitool Bioscience N/A rAAV2-retro-CMV-bGI-Cre-EGFP-pA Taitool Bioscience N/A AAV9.hSyn.GCaMP6m BrainVTA Co.,Ltd.

Techniques: Plasmid Preparation, Recombinant, Software

Journal: Cell reports

Article Title: Lateral hypothalamic LEPR neurons drive appetitive but not consummatory behaviors

doi: 10.1016/j.celrep.2021.109615

Figure Lengend Snippet: (A) Viral injection schematic. (B) Representative images of hM3D, hM4D, and mCherry in the LH of Lepr Cre mice. Scale bar, 500 μm. (C) Chemogenetic LH LEPR manipulations did not affect phase 1 sucrose CPP (post 1), but inhibition blunted the development of CPP following phase 2 training (p = 0.0136). Bonferroni post-tests for LH LEPR :mCherry (**p = 0.0022) and LH LEPR :hM3D mice (**p = 0.0023) in post 2 as compared to pre. Sucrose-side preference was blunted in LH LEPR :hM4D mice as compared to LH LEPR :mCherry (**p = 0.0049) and LH LEPR :hM3D mice (*p = 0.0127). (D) Chemogenetic manipulations did not affect sucrose consumption in either conditioning phase. Each group consumed more sucrose during phase 2 training (****p < 0.0001). (E) Inhibition did not affect the induction of cocaine CPP or reinstatement following extinction. Pre, pre-test; Post, post-test; Ext, extinction test; Rnst, reinstatement test. *p < 0.05, **p < 0.01, ****p < 0.0001. (F) No acute effects of inhibition were observed on the locomotor-stimulating effects of cocaine. (G) LH LEPR inhibition during daily cocaine treatment blunted increases in locomotion. *p = 0.043, **p = 0.0078. (H and I) Prior LH LEPR inhibition attenuated the locomotor-stimulating effects of cocaine following (H) 1 day and (I) 7 days cocaine abstinence; **p < 0.01. Data represented as means ± SEMs; n = 5–10 mice/group. See also for full statistics and .

Article Snippet: Viruses injected include the following: rAAV2/1-EF1α-FLEX-taCasp3-TEVp, titer: 1.9 3 10 12 GC/ml, Addgene 45580, University of North Carolina (UNC) Vector Core (NC, USA) viral prep; rAAV2/1-EF1α-DIO-YFP, titer: 1.9 × 10 12 GC/ml, Addgene 27056, Addgene (MA, USA) 27056-AAV9 viral prep; rAAV2/1-CAG-FLEX-tdTomato, titer: 2.0 × 10 13 GC/ml, Addgene 51503, Addgene 51503-AAV1 viral prep; rAAV2/1-CAG-FLEX-rev-ChR2-tdTomato, titer: 6.8 × 10 12 GC/ml, Addgene 18917, University of Pennsylvania (Penn) Vector Core (PA, USA) viral prep; rAAV2/9-CAG-FLEX-tdTomato, titer: 4.1 × 10 13 GC/ml, Addgene 51503, Penn Vector Core viral prep; rAAV2/5-EF1α-DIO-eNpHR3.0-eYFP, titer: 6 × 10 12 GC/ml, Addgene 26966, UNC Vector Core viral prep; rAAV2/9-CAG-FLEX-ArchT-GFP, titer: 4.7 × 10 12 GC/ml, Addgene 28307, UNC Vector Core viral prep; rAAV2/9-CAG-FLEX-GFP, titer: 2.3 × 10 12 GC/ml, Addgene 51502, Addgene 51502-AAV9 viral prep; rAAV2/9-hSyn-DIO-hM3D(Gq)-mCherry, titer: 5.0 × 10 12 GC/ml, Addgene 44361, Addgene 44361-AAV9 viral prep ( ); rAAV2/9-hSyn-DIO-hM4D(Gi)-mCherry, titer: 5.0 × 10 12 GC/ml, Addgene 44362, Addgene 44362-AAV9 viral prep ( ); rAAV2/9-hSyn-DIO-mCherry, titer: 5.0 × 10 12 GC/ml, Addgene 50459, Addgene 50459-AAV9 viral prep.

Techniques: Injection, Inhibition

Journal: Cell reports

Article Title: Lateral hypothalamic LEPR neurons drive appetitive but not consummatory behaviors

doi: 10.1016/j.celrep.2021.109615

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Viruses injected include the following: rAAV2/1-EF1α-FLEX-taCasp3-TEVp, titer: 1.9 3 10 12 GC/ml, Addgene 45580, University of North Carolina (UNC) Vector Core (NC, USA) viral prep; rAAV2/1-EF1α-DIO-YFP, titer: 1.9 × 10 12 GC/ml, Addgene 27056, Addgene (MA, USA) 27056-AAV9 viral prep; rAAV2/1-CAG-FLEX-tdTomato, titer: 2.0 × 10 13 GC/ml, Addgene 51503, Addgene 51503-AAV1 viral prep; rAAV2/1-CAG-FLEX-rev-ChR2-tdTomato, titer: 6.8 × 10 12 GC/ml, Addgene 18917, University of Pennsylvania (Penn) Vector Core (PA, USA) viral prep; rAAV2/9-CAG-FLEX-tdTomato, titer: 4.1 × 10 13 GC/ml, Addgene 51503, Penn Vector Core viral prep; rAAV2/5-EF1α-DIO-eNpHR3.0-eYFP, titer: 6 × 10 12 GC/ml, Addgene 26966, UNC Vector Core viral prep; rAAV2/9-CAG-FLEX-ArchT-GFP, titer: 4.7 × 10 12 GC/ml, Addgene 28307, UNC Vector Core viral prep; rAAV2/9-CAG-FLEX-GFP, titer: 2.3 × 10 12 GC/ml, Addgene 51502, Addgene 51502-AAV9 viral prep; rAAV2/9-hSyn-DIO-hM3D(Gq)-mCherry, titer: 5.0 × 10 12 GC/ml, Addgene 44361, Addgene 44361-AAV9 viral prep ( ); rAAV2/9-hSyn-DIO-hM4D(Gi)-mCherry, titer: 5.0 × 10 12 GC/ml, Addgene 44362, Addgene 44362-AAV9 viral prep ( ); rAAV2/9-hSyn-DIO-mCherry, titer: 5.0 × 10 12 GC/ml, Addgene 50459, Addgene 50459-AAV9 viral prep.

Techniques: Virus, Plasmid Preparation, Recombinant, Software, Fluorescence, Microscopy, Imaging